SMURF2 Antibody

Recombinant Protein

E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Interacts with SMAD1 and SMAD7 in order to trigger their ubiquitination and proteasome-dependent degradation. In addition, interaction with SMAD7 activates autocatalytic degradation, which is prevented by interaction with SCYE1. Forms a stable complex with the TGF-beta receptor-mediated phosphorylated SMAD2 and SMAD3. In this way, SMAD2 may recruit substrates, such as SNON, for ubiquitin-mediated degradation. Enhances the inhibitory activity of SMAD7 and reduces the transcriptional activity of SMAD2. Coexpression of SMURF2 with SMAD1 results in considerable decrease in steady-state level of SMAD1 protein and a smaller decrease of SMAD2 level.

monoclonal

64750

Purified Mouse Monoclonal Antibody (Mab)

All lanes : Anti-SMURF2 Antibody at 1:2000-1:4000 dilution
Lane 1: MDA-MB-231 whole cell lysate
Lane 2: U-2OS whole cell lysate


Lysates/proteins at 20 µg per lane.

Secondary
Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.

Predicted band size : 86 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

1:2000-1:4000

86196

This SMURF2 antibody is generated from a mouse immunized with a recombinant protein of human SMURF2.