EI2BG Antibody

Recombinant Protein

Catalyzes the exchange of eukaryotic initiation factor 2-bound GDP for GTP.

monoclonal

8891

Purified Mouse Monoclonal Antibody (Mab)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized U-2 OS (human osteosarcoma cell line) cells labeling EI2BG with AM8608b at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-Mouse IgG (174309) secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm staining on U-2 OS cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin (PD18466410) at 1/100 dilution (red).The nuclear counter stain is DAPI (blue).

1:25

WB

Overlay histogram showing U-2 OS cells stained with AM8608b(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AM8608b, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Mouse IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(NH174309) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was mouse IgG2b (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.

1:25

50240

This EI2BG antibody is generated from a mouse immunized with a KLH conjugated synthetic peptide between 110-452 amino acids from human EI2BG.