FZR Antibody

Recombinant Protein

Key regulator of ligase activity of the anaphase promoting complex/cyclosome (APC/C), which confers substrate specificity upon the complex. Associates with the APC/C in late mitosis, in replacement of CDC20, and activates the APC/C during anaphase and telophase. The APC/C remains active in degrading substrates to ensure that positive regulators of the cell cycle do not accumulate prematurely. At the G1/S transition FZR1 is phosphorylated, leading to its dissociation from the APC/C. Following DNA damage, it is required for the G2 DNA damage checkpoint: its dephosphorylation and reassociation with the APC/C leads to the ubiquitination of PLK1, preventing entry into mitosis.

monoclonal

51343

Purified Mouse Monoclonal Antibody (Mab)

All lanes : Anti-FZR Antibody at 1:2000 dilution
Lane 1: 293T/17 whole cell lysate
Lane 2: Hela whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.

Predicted band size : 55 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

1:2000

55179

This FZR antibody is generated from a mouse immunized with a recombinant protein between1-496 amino acids from human FZR.