Item no. |
ABC-AM8494b-ev |
Manufacturer |
Abcepta
|
Amount |
50 ul |
Category |
|
Type |
Antibody Primary |
Format |
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS. |
Applications |
WB, FC, IF, IHC-P |
Specific against |
other |
Host |
Mouse |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Similar products |
VCP, TER ATPase, Transitional endoplasmic reticulum ATPase, TER ATPase, 15S Mg(2+)-ATPase p97 subunit, Valosin-containing protein |
Available |
|
Primary Accession |
P55072 |
Application |
WB, IHC-P, FC, IF |
Bio Background |
Necessary for the fragmentation of Golgi stacks during mitosis and for their reassembly after mitosis. Involved in the formation of the transitional endoplasmic reticulum (tER). The transfer of membranes from the endoplasmic reticulum to the Golgi apparatus occurs via 50-70 nm transition vesicles which derive from part-rough, part-smooth transitional elements of the endoplasmic reticulum (tER). Vesicle budding from the tER is an ATP-dependent process. The ternary complex containing UFD1L, VCP and NPLOC4 binds ubiquitinated proteins and is necessary for the export of misfolded proteins from the ER to the cytoplasm, where they are degraded by the proteasome. The NPLOC4-UFD1L-VCP complex regulates spindle disassembly at the end of mitosis and is necessary for the formation of a closed nuclear envelope. Regulates E3 ubiquitin-protein ligase activity of RNF19A. Component of the VCP/p97-AMFR/gp78 complex that participates in the final step of the sterol-mediated ubiquitination and endoplasmic reticulum-associated degradation (ERAD) of HMGCR. Also involved in DNA damage response: recruited to double-strand breaks (DSBs) sites in a RNF8- and RNF168-dependent manner and promotes the recruitment of TP53BP1 at DNA damage sites. Recruited to stalled replication forks by SPRTN: may act by mediating extraction of DNA polymerase eta (POLH) to prevent excessive translesion DNA synthesis and limit the incidence of mutations induced by DNA damage. Required for cytoplasmic retrotranslocation of stressed/damaged mitochondrial outer-membrane proteins and their subsequent proteasomal degradation. |
Bio References |
Lamerdin J.E., et al.Submitted (MAR-1998) to the EMBL/GenBank/DDBJ databases.Hu R.-M., et al.Proc. Natl. Acad. Sci. U.S.A. 97:9543-9548(2000).Ota T., et al.Nat. Genet. 36:40-45(2004).Humphray S.J., et al.Nature 429:369-374(2004).Mural R.J., et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases. |
Clonality |
monoclonal |
Clone/Animal Names |
1563CT163.48.77 |
Gene ID |
7415 |
Gene Name |
VCP |
Subtitle |
Purified Mouse Monoclonal Antibody (Mab) |
Reactivity |
H, M, Rat |
Legend image 1 |
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervical epithelial adenocarcinoma cell line) cells labeling TERA with AM8494b at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-mouse IgG (NA166821) secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm and nucleus staining on HeLa cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin (PD18466410) at 1/100 dilution (red). |
Type image 1 |
IF |
Dilution image 1 |
1:25 |
Legend image 2 |
All lanes : Anti-VCP Antibody at 1:4000 dilutionLane 1: Hela whole cell lysateLane 2: K562 whole cell lysateLane 3: A549 whole cell lysateLane 4: C6 whole cell lysateLane 5: U-87 MG whole cell lysateLane 6: NIH/3T3 whole cell lysateLysates/proteins at 20 µg per lane. SecondaryGoat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 89 kDaBlocking/Dilution buffer: 5% NFDM/TBST. |
Type image 2 |
WB |
Dilution image 2 |
1:4000 |
Legend image 3 |
AM8494b staining VCP in human breast carcinoma sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody. |
Type image 3 |
IHC-P |
Dilution image 3 |
1:25 |
Legend image 4 |
Overlay histogram showing K562 cells stained with AM8494b (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AM8494b, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Mouse IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(NA168821)) at 1/400 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was mouse IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed. |
Type image 4 |
FC |
Dilution image 4 |
1:25 |
Isotype |
IgG1, κ |
Calculated MW |
89322 |
Antigen Source |
HUMAN |
Target/Specificity |
This VCP antibody is generated from a mouse immunized with a recombinant protein of human TERA. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.