Item no. |
ABC-AM8485b-ev |
Manufacturer |
Abcepta
|
Amount |
50 ul |
Category |
|
Type |
Antibody Primary |
Format |
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS. |
Applications |
WB, IHC-P |
Specific against |
other |
Host |
Mouse |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Similar products |
CDK4, Cyclin-dependent kinase 4, Cell division protein kinase 4, PSK-J3 |
Available |
|
Primary Accession |
P11802 |
Application |
WB, IHC-P |
Bio Background |
Ser/Thr-kinase component of cyclin D-CDK4 (DC) complexes that phosphorylate and inhibit members of the retinoblastoma (RB) protein family including RB1 and regulate the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complexes and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also phosphorylates SMAD3 in a cell-cycle-dependent manner and represses its transcriptional activity. Component of the ternary complex, cyclin D/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex. |
Bio References |
Hanks S.K., et al.Submitted (FEB-1987) to the EMBL/GenBank/DDBJ databases.Elkahloun A.G., et al.Genomics 42:295-301(1997).Wolfel T., et al.Science 269:1281-1284(1995).Zuo L., et al.Nat. Genet. 12:97-99(1996).Ota T., et al.Nat. Genet. 36:40-45(2004). |
Clonality |
monoclonal |
Clone/Animal Names |
1529CT850.162.73 |
Gene ID |
1019 |
Gene Name |
CDK4 |
Subtitle |
Purified Mouse Monoclonal Antibody (Mab) |
Reactivity |
H, M |
Legend image 1 |
Immunohistochemical analysis of paraffin-embedded Human Breast cancer section using Pink1(Cat#AM8485b). AM8485b was diluted at 1:300 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining. |
Type image 1 |
IHC |
Dilution image 1 |
1:300 |
Legend image 2 |
All lanes : Anti-CDK4 Antibody at 1:2000 dilutionLane 1: A431 whole cell lysateLane 2: Hela whole cell lysateLane 3: K562 whole cell lysateLane 4: MCF-7 whole cell lysateLane 5: NIH/3T3 whole cell lysateLysates/proteins at 20 μg per lane. SecondaryGoat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 34 kDaBlocking/Dilution buffer: 5% NFDM/TBST. |
Type image 2 |
WB |
Dilution image 2 |
1:2000 |
Legend image 3 |
AM8485b staining CDK4 in human breast carcinoma sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody. |
Type image 3 |
IHC-P |
Dilution image 3 |
1:25 |
Isotype |
IgG2a, κ |
Calculated MW |
33730 |
Antigen Source |
HUMAN |
Target/Specificity |
This CDK4 antibody is generated from a mouse immunized with a recombinant protein. |
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All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.