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MGMT Antibody

MGMT Antibody

Item no.	ABC-AM8481b-ev
Manufacturer	Abcepta
Amount	50 ul
Category	Antibodies Primary Antibodies
Type	Antibody Primary
Format	Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
Applications	WB, FC
Specific against	other
Host	Mouse
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Similar products	MGMT, Methylated-DNA--protein-cysteine methyltransferase, 6-O-methylguanine-DNA methyltransferase, O-6-methylguanine-DNA-alkyltransferase
Available
Primary Accession	P16455
Application	WB, FC
Bio Background	Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.
Bio References	Tano K., et al.Proc. Natl. Acad. Sci. U.S.A. 87:686-690(1990). Rydberg B., et al.J. Biol. Chem. 265:9563-9569(1990). Koike G., et al.J. Biol. Chem. 265:14754-14762(1990). Hayakawa H., et al.J. Mol. Biol. 213:739-747(1990). Kalnine N., et al.Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
Clonality	monoclonal
Clone/Animal Names	1527CT158.87.44
Gene ID	4255
Gene Name	MGMT
Subtitle	Purified Mouse Monoclonal Antibody (Mab)
Reactivity	H
Legend image 1	All lanes : Anti-MGMT Antibody at1:1000-1:2000 dilution Lane 1: Jurkat whole cell lysate Lane 2: MCF-7 whole cell lysate Lane 3: MOLT-4 whole cell lysate Lane 4: Raji whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 22 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Type image 1	WB
Dilution image 1	1:1000-1:2000
Legend image 2	Overlay histogram showing Jurkat cells stained with AM8481b (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AM8481b, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-mouse IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(NA168821)) at 1/400 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was mouse IgG (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
Type image 2	FC
Dilution image 2	1:25
Isotype	IgG1, k
Calculated MW	21646
Antigen Source	HUMAN
Target/Specificity	This MGMT antibody is generated from a mouse immunized with a recombinant protein.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 50 ul

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Delivery expected until 9/6/2024