Item no. |
ABC-AM2199b-ev |
Manufacturer |
Abcepta
|
Amount |
50 ul |
Category |
|
Type |
Antibody Primary |
Format |
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS. |
Applications |
WB, IHC-P |
Specific against |
other |
Host |
Mouse |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Similar products |
H2AFX, H2AX, H2a/x, Histone H2AX |
Available |
|
Primary Accession |
P16104 |
Antigen Type |
Synthetic Peptide |
Application |
IHC-P, WB |
Bio Background |
Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation. |
Bio References |
Stewart G.S., et al. Nature 421:961-966(2003).Park E.-J., et al. Nucleic Acids Res. 31:6819-6827(2003).Stiff T., et al. Cancer Res. 64:2390-2396(2004).Lukas C., et al. EMBO J. 23:2674-2683(2004).Kurz E.U., et al. J. Biol. Chem. 279:53272-53281(2004). |
Clonality |
Monoclonal |
Clone/Animal Names |
938CT5.1.1 |
Gene ID |
3014 |
Gene Name |
|
Subtitle |
Mouse Monoclonal Antibody (Mab) |
Reactivity |
H |
Legend image 1 |
Western blot analysis of lysates from 293, CEM, HepG2, Jurkat, Hela, Raji cell line(from left to right). using H2AFX Antibody (C-term)(Cat. #AM2199b). AM2199b was diluted at 1:2000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:3000 dilution was used as the secondary antibody. Lysates at 35μg per lane. |
Type image 1 |
WB |
Dilution image 1 |
1:1000 |
Legend image 2 |
Immunohistochemical analysis of paraffin-embedded Human Thymus section using Pink1(Cat#am2199b). am2199b was diluted at 1:50 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining. |
Type image 2 |
IHC |
Dilution image 2 |
1:50 |
Legend image 3 |
Immunohistochemical analysis of paraffin-embedded H. prostate section using H2AFX Antibody (C-term)(Cat#AM2199b). AM2199b was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining. |
Type image 3 |
IHC-P |
Dilution image 3 |
1:25 |
Legend image 4 |
Immunohistochemical analysis of paraffin-embedded H. thymus section using H2AFX Antibody (C-term)(Cat#AM2199b). AM2199b was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining. |
Type image 4 |
IHC-P |
Dilution image 4 |
1:25 |
Isotype |
IgG1 |
Calculated MW |
15145 |
Antigen Region |
115-143 |
Target/Specificity |
This H2AFX antibody is generated from mice immunized with a KLH conjugated synthetic peptide between 115-143 amino acids from the C-terminal region of human H2AFX. |
NCBI Accession |
NP_002096.1 |
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All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.