Item no. |
ABC-AM2067B |
Manufacturer |
Abcepta
|
Amount |
400 ul |
Category |
|
Type |
Antibody Primary |
Format |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
Applications |
WB, IF |
Specific against |
other |
Host |
Mouse |
Isotype |
IgG1 kappa |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Similar products |
FAS, FASN, Fatty acid synthase, [Acyl-carrier-protein] S-acetyltransferase, [Acyl-carrier-protein] S-malonyltransferase, 3-oxoacyl-[acyl-carrier-protein] synthase, 3-oxoacyl-[acyl-carrier-protein] reductase, 3-hydroxyacyl-[acyl-carrier-protein] dehydratase, Enoyl-[acyl-carrier-protein] reductase, Oleoyl-[acyl-carrier-protein] hydrolase |
Available |
|
Primary Accession |
P49327 |
Antigen Type |
Synthetic Peptide |
Application |
WB, IF |
Bio Background |
The enzyme encoded by this gene is a multifunctionalprotein. Its main function is to catalyze the synthesis ofpalmitate from acetyl-CoA and malonyl-CoA, in the presence ofNADPH, into long-chain saturated fatty acids. In some cancer celllines, this protein has been found to be fused with estrogenreceptor-alpha (ER-alpha), in which the N-terminus of FAS is fusedin-frame with the C-terminus of ER-alpha. |
Bio References |
References for protein:1.Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)2.Nguyen, P.L., et al. J. Clin. Oncol. 28(25):3958-3964(2010)3.Ruano, G., et al. Pharmacogenomics 11(7):959-971(2010)4.Tischler, V., et al. Histopathology 56(6):811-815(2010)5.Dorn, C., et al. Int J Clin Exp Pathol 3(5):505-514(2010)References for HepG2 cell line:1. Knowles BB, et al. (1980). Human hepatocellular carcinoma cell lines secrete the major plasma proteins and hepatitis B surface antigen. Science 209: 497-499.[ PubMed: 6248960].2. Darlington GJ, et al. (1987). Growth and hepatospecific gene expression of human hepatoma cells in a defined medium. In Vitro Cell. Dev. Biol. 23: 349-354.[PubMed: 3034851].3. Ihrke, G; Neufeld, EB; Meads, T; Shanks, MR; Cassio, D; Laurent, M; Schroer, TA; Pagano, RE et al. (1993). "WIF-B cells: an in vitro model for studies of hepatocyte polarity". Journal of Cell Biology 123 (6): 1761–1775. [PubMed:7506266]. 4. Mersch-Sundermann, V.; Knasmüller, S.; Wu, X. J.; Darroudi, F.; Kassie, F. (2004). "Use of a human-derived liver cell line for the detection of cytoprotective, antigenotoxic and cogenotoxic agents". Toxicology 198 (1–3): 329–340. [PubMed:15138059]. |
Clonality |
Monoclonal |
Clone/Animal Names |
497CT15.2.5 |
Gene ID |
2194 |
Gene Name |
FASN |
Subtitle |
Mouse Monoclonal Antibody (Mab) |
Reactivity |
H |
Legend image 1 |
All lanes : Anti-FASN Antibody (Center) at 1:500-1:2000 dilutionLane 1: A549 whole cell lysateLane 2: Hela whole cell lysateLane 3: 293 whole cell lysateLane 4: Ramos whole cell lysateLane 5: HepG2 whole cell lysateLysates/proteins at 20 µg per lane. SecondaryGoat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 273 kDaBlocking/Dilution buffer: 5% NFDM/TBST. |
Type image 1 |
WB |
Dilution image 1 |
1:500-1:2000 |
Legend image 2 |
Fluorescent confocal image of HepG2 cells stained with FASN (Center) antibody. HepG2 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AM2067b FASN primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-mouse antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min). Note the highly specific localization of the FASN immunosignal to the cytoplasm, supported by Human Protein Atlas Data (http://www.proteinatlas.org/ENSG00000169710). |
Type image 2 |
IF |
Dilution image 2 |
1:200 |
Legend image 3 |
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized U-2 OS ((human cervical epithelial adenocarcinoma cell line) cells labeling FASN with AM2067b at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-mouse IgG (35503) secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm Hela cell line. The nuclear counter stain is DAPI (blue). |
Type image 3 |
IF |
Dilution image 3 |
1:25 |
Legend image 4 |
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized HepG2 (human liver hepatocellular carcinoma cell line) cells labeling FASN with AM2067b at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-mouse IgG (35503) secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm HepG2 cell line. The nuclear counter stain is DAPI (blue). |
Type image 4 |
IF |
Dilution image 4 |
1:25 |
Legend image 5 |
FASN Antibody (Center) (Cat. #AM2067b) western blot analysis in mouse brain tissue lysates (35μg/lane).This demonstrates the FASN (Center) antibody detected the FASN (Center) protein (arrow). |
Type image 5 |
WB |
Dilution image 5 |
1:100 |
Legend image 6 |
All lanes : Anti-FASN Antibody (Center) at 1:8000 dilutionLane 1: A549 whole cell lysateLane 2: Hela whole cell lysateLane 3: 293 whole cell lysateLane 4: Ramos whole cell lysateLane 5: HepG2 whole cell lysateLysates/proteins at 20 µg per lane. SecondaryGoat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 273 kDaBlocking/Dilution buffer: 5% NFDM/TBST. |
Type image 6 |
WB |
Dilution image 6 |
1:8000 |
Legend image 7 |
Anti- at 1:1000 dilution + HepG2 whole cell lysateLysates/proteins at 20 µg per lane. SecondaryGoat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 273 kDaBlocking/Dilution buffer: 5% NFDM/TBST. |
Type image 7 |
WB |
Dilution image 7 |
1:1000 |
Other Accession |
NP_004095.4 |
Isotype |
IgG1, κ |
Calculated MW |
273427 |
Antigen Region |
942-973 |
Antigen Source |
HUMAN |
Target/Specificity |
This FASN antibody is generated from mice immunized with a KLH conjugated synthetic peptide between 942-973 amino acids from the Central region of human FASN. |
NCBI Accession |
NP_004095.4 |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.