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Goat Anti-PTBP1 / PTB Antibody

Goat Anti-PTBP1 / PTB Antibody

Manufacturer	Abcepta
Category	Antibodies Primary Antibodies
Type	Antibody Primary
Specific against	other
Applications	WB, IHC, ELISA
Amount	100 ug
Host	Goat
Item no.	ABC-AF1877a
eClass 6.1	32160702
eClass 9.0	32160702
Available
Primary Accession	P17225.2
Antigen Type	Synthetic Peptide
Application	WB, IHC, E
Bio Background	This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA-binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has four repeats of quasi-RNA recognition motif (RRM) domains that bind RNAs. This protein binds to the intronic polypyrimidine tracts that requires pre-mRNA splicing and acts via the protein degradation ubiquitin-proteasome pathway. It may also promote the binding of U2 snRNP to pre-mRNAs. This protein is localized in the nucleoplasm and it is also detected in the perinucleolar structure. Alternatively spliced transcript variants encoding different isoforms have been described.
Bio References	Upregulated c-myc expression in multiple myeloma by internal ribosome entry results from increased interactions with and expression of PTB-1 and YB-1. Cobbold LC, et al. Oncogene, 2010 May 13. PMID 20190818. Interactions between PTB RRMs induce slow motions and increase RNA binding affinity. Maynard CM, et al. J Mol Biol, 2010 Mar 19. PMID 20080103. Polypyrimidine tract-binding protein interacts with coxsackievirus B3 RNA and influences its translation. Verma B, et al. J Gen Virol, 2010 May. PMID 20071487. Genome-wide analysis of PTB-RNA interactions reveals a strategy used by the general splicing repressor to modulate exon inclusion or skipping. Xue Y, et al. Mol Cell, 2009 Dec 25. PMID 20064465. Polypyrimidine tract binding proteins (PTB) regulate the expression of apoptotic genes and susceptibility to caspase-dependent apoptosis in differentiating cardiomyocytes. Zhang J, et al. Cell Death Differ, 2009 Nov. PMID 19590510.
Clonality	Polyclonal
Subtitle	Peptide-affinity purified goat antibody
Reactivity	H
Legend image 1	AF1877a (0.1 µg/ml) staining of Human Ovary lysate (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Type image 1	WB
Legend image 2	AF1877a (0.01 µg/ml) staining of HeLa (A), HepG2 (B), Jurkat (C) and HEK293 (D) nuclear lysates (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Type image 2	WB
Legend image 3	AF1877a (2µg/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.
Type image 3	IHC
Other Accession	NP_787041; 19205 (mouse); 29497 (rat);
Predicted	M, Rat, Pig, Cow, Dog
Concentration (mg/ml)	100ug/200ul

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 100 ug

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Delivery expected until 9/6/2024

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