Comparison

Functional Angiopoietin-2 Antibody,mAb (recombinant) (blocking)(preservative free)

Item no. ABC-ADP0007-U0100
Manufacturer Abcepta
Amount 100 ug
Category
Type Antibody Primary
Applications ELISA
Clone Angy-2-1
Specific against other
Host CHO cells
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Similar products Ang-2; Ang2; Angpt2; Agpt2
Available
Primary Accession
O35608
Application
E
Bio Background
Angiopoietin-1 (Ang-1) and Angiopoietin-2 (Ang-2) are closely related secreted ligands which bind with similar affinity to Tie-2. Tie-2 and angiopoietins have been shown to play critical roles in embryogenic angiogenesis and in maintaining the integrity of the adult vasculature. Ang-1 activates Tie-2 signaling on endothelial cells to promote chemotaxis, cell survival, cell sprouting, vessel growth and stabilization. Ang-2 has been identified as a secreted protein ligand of Tie-2 and has alternatively been reported to be an antagonist for Ang-1 induced Tie-2 signaling as well as an agonist for Tie-2 signaling, depending on the cell context.

anti-Angiopoietin-2, mAb (rec.) (blocking) (Angy-2-1) is an antibody developed by antibody phage display technology using a human naive antibody gene library. These libraries consist of scFv (single chain fragment variable) composed of VH (variable domain of the human immunoglobulin heavy chain) and VL (variable domain of the human immunoglobulin light chain) connected by a polypeptide linker. The antibody fragments are displayed on the surface of filamentous bacteriophage (M13). This scFv was selected by affinity selection on antigen in a process termed panning. Multiple rounds of panning are performed to enrich for antigen-specific scFv-phage. Monoclonal antibodies are subsequently identified by screening after each round of selection. The selected monoclonal scFv is cloned into an appropriate vector containing a Fc portion of interest and then produced in mammalian cells to generate an IgG like scFv-Fc fusion protein.
Clonality
Monoclonal
Gene ID
11601
Gene Name
Angpt2
Reactivity
H, M
Legend image 1
Binding of human Angiopoietin-2 to Tie-2 (human):Fc is inhibited by the antibody anti-Angiopoietin-2, mAb (rec.) (blocking) (Angy-2-1)).
Methods:Tie-2 (h):Fc was coated on an ELISA plate at 1 µg/ml. anti-Angiopoietin-2, mAb (rec.) (blocking) (Angy-2-1) or an unrelated mAb (recombinant) (Control) were added (starting at 40 µg/ml with a twofold serial dilution) together with 20ng/ µl of Angiopoietin-2 (human). After incubation for 1h at RT, the binding was detected using an anti-FLAG antibody (HRP).
Type image 1
E
Clone: Angy-2-1
Concentration (mg/ml)
1mg/ml

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 100 ug
Available: In stock
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