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Anti-Nucleophosmin Picoband Antibody

Anti-Nucleophosmin Picoband Antibody

Manufacturer	Abcepta
Category	Antibodies Primary Antibodies
Type	Antibody Primary
Specific against	other
Format	Lyophilized
Applications	WB, IHC-P
Amount	100 ug
Host	Rabbit
Item no.	ABC-ABO12032
eClass 6.1	32160702
eClass 9.0	32160702
Available
Primary Accession	P06748
Application	WB, IHC-P
Clonality	Polyclonal
Gene ID	4869
Reactivity	H, M, Rat
Legend image 1	Anti- Nucleophosmin Picoband antibody, ABO12032, Western blottingAll lanes: Anti Nucleophosmin (ABO12032) at 0.5ug/mlLane 1: Rat Testis Tissue Lysate at 50ugLane 2: Mouse Testis Tissue Lysate at 50ugLane 3: SKOV Whole Cell Lysate at 40ugLane 4: HEPA Whole Cell Lysate at 40ugLane 5: JURKAT Whole Cell Lysate at 40ugLane 6: HUT Whole Cell Lysate at 40ugLane 7: HELA Whole Cell Lysate at 40ugPredicted bind size: 33KDObserved bind size: 38KD
Type image 1	WB
Dilution image 1	0.1-0.5 µg/ml
Legend image 2	Anti- Nucleophosmin Picoband antibody, ABO12032, IHC(P)IHC(P): Mouse Intestine Tissue
Type image 2	IHC
Dilution image 2	0.5-1 µg/ml
Legend image 3	Anti- Nucleophosmin Picoband antibody, ABO12032, IHC(P)IHC(P): Rat Intestine Tissue
Type image 3	IHC
Legend image 4	Anti- Nucleophosmin Picoband antibody, ABO12032, IHC(P)IHC(P): Human Intestinal Cancer Tissue
Type image 4	IHC
Calculated MW	32575
Reconstitution	Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Description	Rabbit IgG polyclonal antibody for Nucleophosmin(NPM1) detection. Tested with WB, IHC-P in Human; Mouse; Rat.
Protein Function	Involved in diverse cellular processes such as ribosome biogenesis, centrosome duplication, protein chaperoning, histone assembly, cell proliferation, and regulation of tumor suppressors p53/TP53 and ARF. Binds ribosome presumably to drive ribosome nuclear export. Associated with nucleolar ribonucleoprotein structures and bind single-stranded nucleic acids. Acts as a chaperonin for the core histones H3, H2B and H4. Stimulates APEX1 endonuclease activity on apurinic/apyrimidinic (AP) double- stranded DNA but inhibits APEX1 endonuclease activity on AP single-stranded RNA. May exert a control of APEX1 endonuclease activity within nucleoli devoted to repair AP on rDNA and the removal of oxidized rRNA molecules. In concert with BRCA2, regulates centrosome duplication. Regulates centriole duplication: phosphorylation by PLK2 is able to trigger centriole replication. Negatively regulates the activation of EIF2AK2/PKR and suppresses apoptosis through inhibition of EIF2AK2/PKR autophosphorylation. .
Subcellular Localization	Nucleus, nucleolus. Nucleus, nucleoplasm. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Generally nucleolar, but is translocated to the nucleoplasm in case of serum starvation or treatment with anticancer drugs. Has been found in the cytoplasm in patients with primary acute myelogenous leukemia (AML), but not with secondary AML. Can shuttle between cytoplasm and nucleus. Co- localizes with the methylated form of RPS10 in the granular component (GC) region of the nucleolus. Colocalized with nucleolin and APEX1 in nucleoli. Isoform 1 of NEK2 is required for its localization to the centrosome during mitosis.
Protein Name	Nucleophosmin
Contents	Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Immunogen	E.coli-derived human Nucleophosmin recombinant protein (Position: M1-L294). Human Nucleophosmin shares 95% amino acid (aa) sequence identity with both mouse and rat Nucleophosmin.
Purification	Immunogen affinity purified.
Cross Reactivity	No cross reactivity with other proteins
Storage	At -20?C for one year. After r?Constitution, at 4?C for one month. It?Can also be aliquotted and stored frozen at -20?C for a longer time.Avoid repeated freezing and thawing.
Concentration (mg/ml)	Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Sequence Similarities	Belongs to the nucleoplasmin family.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 100 ug

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