Anti-AIF Antibody

WB, IHC-P, IHC-F, ICC

9131

Anti-AIF antibody, ABO11542, IHC(P)IHC(P): Human Lung Cancer Tissue

0.5-1 µg/ml

IHC

Anti-AIF antibody, ABO11542, Western blottingLane 1: Rat Heart Tissue LysateLane 2: Rat Brain Tissue LysateLane 3: K562 Cell LysateLane 4: HEPG2 Cell LysateLane 5: A431 Cell LysateLane 6: NIH3T3 Cell Lysate

0.1-0.5 µg/ml

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Functions both as NADH oxidoreductase and as regulator of apoptosis. In response to apoptotic stimuli, it is released from the mitochondrion intermembrane space into the cytosol and to the nucleus, where it functions as a proapoptotic factor in a caspase-independent pathway. In contrast, functions as an antiapoptotic factor in normal mitochondria via its NADH oxidoreductase activity. The soluble form (AIFsol) found in the nucleus induces 'parthanatos' i.e. caspase-independent fragmentation of chromosomal DNA. Interacts with EIF3G, and thereby inhibits the EIF3 machinery and protein synthesis, and activates casapse-7 to amplify apoptosis. Plays a critical role in caspase- independent, pyknotic cell death in hydrogen peroxide-exposed cells. Binds to DNA in a sequence-independent manner. .

Detected in muscle and skin fibroblasts (at protein level). Isoform 5 is frequently down-regulated in human cancers. .

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Immunogen affinity purified.

At -20?C for one year. After r?Constitution, at 4?C for one month. It?Can also be aliquotted and stored frozen at -20?C for a longer time.Avoid repeated freezing and thawing.

Belongs to the FAD-dependent oxidoreductase family.