Comparison

Anti-Collagen II Antibody

Item no. ABC-ABO11451
Manufacturer Abcepta
Amount 100 ug
Category
Type Antibody Primary
Format Lyophilized
Applications WB
Specific against other
Host Rabbit
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Similar products COL2A1, Chondrocalcin, Collagen alpha-1(II) chain, Alpha-1 type II collagen
Available
Primary Accession
P02458
Application
WB
Clonality
Polyclonal
Gene ID
1280
Reactivity
H, M, Rat
Legend image 1
Anti- COL2A1 antibody, ABO11451, Western blottingAll lanes: Anti COL2A1 (ABO11451) at 0.5ug/mlLane 1: COLO320 Whole Cell Lysate at 40ugLane 2: HELA Whole Cell Lysate at 40ugLane 3: MCF-7Whole Cell Lysate at 40ugLane 4: A549 Whole Cell Lysate at 40ugLane 5: JURKAT Whole Cell Lysate at 40ugPredicted bind size: 134 KDObserved bind size: 170KD
Type image 1
WB
Dilution image 1
0.1-0.5 µg/ml
Calculated MW
141785
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Description
Rabbit IgG polyclonal antibody for Collagen alpha-1(II) chain(COL2A1) detection. Tested with WB in Human; Mouse; Rat.
Protein Function
Type II collagen is specific for cartilaginous tissues. It is essential for the normal embryonic development of the skeleton, for linear growth and for the ability of cartilage to resist compressive forces.
Subcellular Localization
Secreted, extracellular space, extracellular matrix .
Tissue Specificity
Isoform 2 is highly expressed in juvenile chondrocyte and low in fetal chondrocyte. .
Protein Name
Collagen alpha-1(II) chain
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Collagen II(1222-1241aa AFAGLGPREKGPDPLQYMRA), different from the related rat sequence by one amino acid, and from the related mouse sequence by two amino acids.
Purification
Immunogen affinity purified.
Cross Reactivity
No cross reactivity with other proteins
Storage
At -20?C for one year. After r?Constitution, at 4?C for one month. It?Can also be aliquotted and stored frozen at -20?C for a longer time.Avoid repeated freezing and thawing.
Concentration (mg/ml)
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Sequence Similarities
Belongs to the fibrillar collagen family.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 100 ug
Available: In stock
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