Item no. |
ABC-ABO10584 |
Manufacturer |
Abcepta
|
Amount |
100 ug |
Category |
|
Type |
Antibody Primary |
Format |
Lyophilized |
Applications |
WB |
Specific against |
other |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Similar products |
Endothelial PAS domain-containing protein 1, Hypoxia-inducible factor 2-alpha, EPAS-1, Epas1, Hif2a, HIF-2-alpha, HIF2-alpha |
Available |
|
Primary Accession |
Q9JHS1 |
Application |
WB |
Clonality |
Polyclonal |
Gene ID |
29452 |
Reactivity |
H, M, Rat |
Legend image 1 |
Figure 1. Western blot analysis of HIF2A using anti- HIF2A antibody (ABO10584). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat thymus tissue lysates, Lane 2: rat testis tissue lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- HIF2A antigen affinity purified polyclonal antibody (Catalog # ABO10584) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for HIF2A at approximately 110-120KD. The expected band size for HIF2A is at 96KD. |
Type image 1 |
WB |
Dilution image 1 |
0.1-0.5 µg/ml |
Calculated MW |
96718 |
Reconstitution |
Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
Description |
Rabbit IgG polyclonal antibody for Endothelial PAS domain-containing protein 1(EPAS1) detection. Tested with WB in Human; Mouse; Rat. |
Protein Function |
Transcription factor involved in the induction of oxygen regulated genes. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Regulates the vascular endothelial growth factor (VEGF) expression and seems to be implicated in the development of blood vessels and the tubular system of lung. May also play a role in the formation of the endothelium that gives rise to the blood brain barrier. Potent activator of the Tie-2 tyrosine kinase expression. Activation seems to require recruitment of transcriptional coactivators such as CREBPB and probably EP300. Interaction with redox regulatory protein APEX seems to activate CTAD (By similarity). . |
Subcellular Localization |
Nucleus . Nucleus speckle . Colocalizes with HIF3A in the nucleus and speckles. . |
Protein Name |
Endothelial PAS domain-containing protein 1(EPAS-1) |
Contents |
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. |
Immunogen |
A synthetic peptide corresponding to a sequence in the middle region of human HIF-2-alpha(282-301aa, HALDSENMTKSHQNLCTKGQ), identical to the related mouse and rat sequence. |
Purification |
Immunogen affinity purified. |
Cross Reactivity |
No cross reactivity with other proteins |
Storage |
At -20?C for one year. After r?Constitution, at 4?C for one month. It?Can also be aliquotted and stored frozen at -20?C for a longer time.Avoid repeated freezing and thawing. |
Concentration (mg/ml) |
Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
Sequence Similarities |
Contains 1 bHLH (basic helix-loop-helix) domain. |
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All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.