Anti-RanBP1 Picoband Antibody

WB, IHC-P

5902

Figure 1. Western blot analysis of RanBP1 using anti- RanBP1 antibody (ABO10300). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: MCF-7 whole Cell lysates, Lane 2: HELA whole Cell lysates, Lane 3: HEPG2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- RanBP1 antigen affinity purified polyclonal antibody (Catalog # ABO10300) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for RanBP1 at approximately 27KD. The expected band size for RanBP1 is at 23KD.

0.1-0.5 µg/ml

IHC

Figure 3. IHC analysis of RanBP1 using anti- RanBP1 antibody (ABO10300).RanBP1 was detected in paraffin-embedded section of rat intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti- RanBP1 Antibody (ABO10300) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

Figure 4. IHC analysis of RanBP1 using anti- RanBP1 antibody (ABO10300).RanBP1 was detected in paraffin-embedded section of human intestinal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti- RanBP1 Antibody (ABO10300) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

Figure 5. IHC analysis of RanBP1 using anti- RanBP1 antibody (ABO10300).RanBP1 was detected in paraffin-embedded section of human lung cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti- RanBP1 Antibody (ABO10300) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

23310

Rabbit IgG polyclonal antibody for Ran-specific GTPase-activating protein(RANBP1) detection. Tested with WB, IHC-P in Human; Mouse; Rat.

Ran-specific GTPase-activating protein

E.coli-derived human RanBP1 recombinant protein (Position: A2-Q201). Human RanBP1 shares 93.1% amino acid (aa) sequence identity with mouse RanBP1.

No cross reactivity with other proteins.

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.