Item no. |
ABC-ABO10277 |
Manufacturer |
Abcepta
|
Amount |
100 ug |
Category |
|
Type |
Antibody Primary |
Format |
Lyophilized |
Applications |
WB |
Specific against |
other |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Similar products |
E2F3, KIAA0075, Transcription factor E2F3, E2F-3 |
Available |
|
Primary Accession |
O00716 |
Application |
WB |
Clonality |
Polyclonal |
Gene ID |
1871 |
Reactivity |
H, M, Rat |
Legend image 1 |
Western blot analysis of E2F3 expression in rat liver extract (lane 1), mouse cardiac muscle extract (lane 2) and U2OS whole cell lysates (lane 3). E2F3 at 49KD was detected using rabbit anti- E2F3 Antigen Affinity purified polyclonal antibody (Catalog # ABO10277) at 0.5 μg/mL. The blot was developed using chemiluminescence (ECL) method . |
Type image 1 |
WB |
Dilution image 1 |
0.1-0.5 µg/ml |
Calculated MW |
49162 |
Reconstitution |
Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
Description |
Rabbit IgG polyclonal antibody for Transcription factor E2F3(E2F3) detection. Tested with WB in Human; Mouse; Rat. |
Protein Function |
Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC- 3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F3 binds specifically to RB1 in a cell-cycle dependent manner. Inhibits adipogenesis, probably through the repression of CEBPA binding to its target gene promoters (By similarity). . |
Subcellular Localization |
Nucleus. |
Protein Name |
Transcription factor E2F3 |
Contents |
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3. |
Immunogen |
A synthetic peptide corresponding to a sequence at the C-terminus of human E2F3 (278-320aa QSCTLDLKLLTEDSENQRLAYVTYQDIRKISGLKDQTVIVVKA), identical to the related mouse sequence. |
Purification |
Immunogen affinity purified. |
Cross Reactivity |
No cross reactivity with other proteins. |
Storage |
At -20?C for one year. After r?Constitution, at 4?C for one month. It?Can also be aliquotted and stored frozen at -20?C for a longer time.Avoid repeated freezing and thawing. |
Concentration (mg/ml) |
Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.