Item no. |
ABC-ABO10045 |
Manufacturer |
Abcepta
|
Amount |
100 ug |
Category |
|
Type |
Antibody Primary |
Format |
Lyophilized |
Applications |
WB, ELISA, IHC-P |
Specific against |
other |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Similar products |
Ptx1, C-reactive protein, Crp |
Available |
|
Primary Accession |
P14847 |
Application |
WB, IHC-P, E |
Clonality |
Polyclonal |
Gene ID |
12944 |
Reactivity |
M, Rat |
Legend image 1 |
Figure 1. Western blot analysis of Crp using anti-Crp antibody (ABO10045). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat spleen tissue lysates, Lane 2: rat brain tissue lysates, Lane 3: rat thymus tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Crp antigen affinity purified polyclonal antibody (Catalog # ABO10045) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Crp at approximately 25KD. The expected band size for Crp is at 25KD. |
Type image 1 |
WB |
Dilution image 1 |
0.1-0.5 µg/ml |
Legend image 2 |
Figure 2. IHC analysis of Crp using anti- Crp antibody (ABO10045).Crp was detected in paraffin-embedded section of mouse kidney tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti- Crp Antibody (ABO10045) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen. |
Type image 2 |
IHC |
Dilution image 2 |
0.5-1 µg/ml |
Legend image 3 |
Figure 3. IHC analysis of Crp using anti- Crp antibody (ABO10045).Crp was detected in paraffin-embedded section of rat kidney tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti- Crp Antibody (ABO10045) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen. |
Type image 3 |
IHC |
Calculated MW |
25360 |
Reconstitution |
Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
Description |
Rabbit IgG polyclonal antibody for C-reactive protein(Crp) detection. Tested with WB, IHC-P, ELISA in Mouse; Rat. |
Protein Function |
Displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells. |
Subcellular Localization |
Secreted. |
Tissue Specificity |
Found in plasma. |
Protein Name |
C-reactive protein |
Contents |
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3. |
Immunogen |
E. coli-derived mouse Crp recombinant protein (Position: H20-S225). Mouse Crp shares 71.9% and 72.7% amino acid (aa) sequence identity with human and rat Crp, respectively. |
Purification |
Immunogen affinity purified. |
Cross Reactivity |
No cross reactivity with other proteins |
Storage |
At -20?C for one year. After r?Constitution, at 4?C for one month. It?Can also be aliquotted and stored frozen at -20?C for a longer time.Avoid repeated freezing and thawing. |
Concentration (mg/ml) |
Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.