Comparison

Biotinylated Anti-Rat Leptin Antibody

Item no. ABC-ABG10344-U025
Manufacturer Abcepta
Amount 25 ug
Category
Type Antibody Primary
Applications WB, ELISA
Specific against other
Host Goat
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Available
Application
WB, E
Clonality
Polyclonal
Reactivity
Rat
Preparation
Produced from sera of goats pre-immunized with highly pure (> 98%) recombinant Rat Leptin. Anti-Rat Leptin specific antibody was purified by affinity chromatography and then biotinylated.
WesternBlot
To detect Rat Leptin by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µ g/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat Leptin is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Sandwich
To detect Rat Leptin by sandwich ELISA (using 100 μ l/well antibody solution) a concentration of 0.25 – 1.0 μ g/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with BioGems' Polyclonal Anti-Rat Leptin (62-063P) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant Rat Leptin.
Direct
To detect Rat Leptin by direct ELISA (using 100 μ l/well antibody solution) a concentration of 0.25 – 1.0 μ g/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 – 0.4 ng/well of recombinant Rat Leptin.
Formulation
A sterile filtered antibody solution was lyophilized from PBS, pH 7.2.
Reconstitution
Centrifuge vial prior to opening. Reconstitute in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 25 ug
Available: In stock
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