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Anti-Rat IP-10 Antibody

Anti-Rat IP-10 Antibody

Item no.	ABC-ABG10317-U050
Manufacturer	Abcepta
Amount	50 ug
Category	Antibodies Primary Antibodies
Type	Antibody Primary
Applications	WB, IHC, ELISA
Specific against	other
Host	Rabbit
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Available
Application	WB, IHC, E
Clonality	Polyclonal
Reactivity	Rat
Preparation	Produced from sera of rabbits immunized with highly pure recombinant Rat IP-10. Anti-Rat IP-10 specific antibody was purified by affinity chromatography employing an immobilized Rat IP-10 matrix.
WesternBlot	To detect Rat IP-10 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ g/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat IP-10 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Sandwich	To detect Rat IP-10 by sandwich ELISA (using 100μ l/well antibody solution) a concentration of 0.5 - 2.0 μ g/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with BioGems' Biotinylated Anti-Rat IP-10 (62-062BT) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat IP-10.
Immunohistochemistry	This antibody stained colchicine injected rat brain (including the caudate putamen) tissue. The primary antibody was incubated at 0.25 μ g/ml overnight at 4?C. This was followed by a peroxidase conjugated secondary antibody and then a fluorescein Tyramide Signal Amplification (TSA™ ) reagent. Optimal concentrations and conditions may vary. *Information and photo are courtesy of the Tissue Profiling group, SciLifeLab Stockholm.*
Neutralization	To yield one-half maximal inhibition [ND₅₀] of the biological activity of Rat IP-10 (100 ng/ml), a concentration of 5.0-10.0 μ g/ml of this antibody is required.
Formulation	A sterile filtered antibody solution was lyophilized from PBS, pH 7.2.
Reconstitution	Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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