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Anti-Human BMP-7 Antibody

Anti-Human BMP-7 Antibody

Item no.	ABC-ABG10040-U500
Manufacturer	Abcepta
Amount	500 ug
Category	Antibodies Primary Antibodies
Type	Antibody Primary
Applications	WB, IHC, ELISA
Specific against	other
Host	Mouse
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Available
Application	WB, IHC, E
Clonality	Monoclonal
Reactivity	H
Preparation	Produced in mice using highly pure (> 98%) recombinant human BMP-7/OP-1 as the immunizing antigen. This IgG1_K antibody was purified from cell culture by Protein G affinity chromatography.
WesternBlot	To detect hBMP-7 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µ g/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hBMP-7 is 1.5 - 3.1 ng/lane, under either reducing
Sandwich	In a sandwich ELISA (assuming 100µ l/well), when used as a capture antibody at a concentration of 2.0-4.0 µ g/ml, this antibody will detect at least 100 pg/ml of recombinant human BMP-7/OP-1 when used with BioGems' biotinylated antigen-affinity purified anti-hBMP-7/OP-1 (60-088BT) as the detection antibody at a concentration of approximately 0.5-1.0 µ g/ml.
Immunohistochemistry	This antibody stained formalin-fixed, paraffin-embedded sections of human breast malignant carcinoma. The recommended concentration is 5.0 µ g/mL with an overnight incubation at 4?C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 9.0 buffer is recommended. See photo(s). Optimal concentrations and conditions may vary. Tissue samples were provided by the Cooperative Human Tissue Network, which is funded by the National Cancer Institute. Information and photo are courtesy of Flagship Biosciences, LLC – Histology Services.
Formulation	A sterile filtered antibody solution was lyophilized from PBS.
Reconstitution	Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 500 ug

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