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Peroxidase-conjugated AffiniPure® F(ab')2 Fragment Goat Anti-Mouse IgG, Fcγ Fragment Specific

Peroxidase-conjugated AffiniPure® F(ab')2 Fragment Goat Anti-Mouse IgG, Fcγ Fragment Specific

Item no.	115-036-008
Manufacturer	Jackson ImmunoResearch
Amount	0,5 ml
Category	Antibodies Secondary Antibodies
Type	Antibody Polyclonal
Format	Lyophilized
Specific against	Mouse (Murine, Mus musculus)
Host	Caprine
Conjugate/Tag	Horseradish Peroxidase
Purity	The antibody was purified from antisera by a combination of pepsin digestion and immunoaffinity chromatography using antigens coupled to agarose beads. Fc fragments and whole IgG molecules have been removed.
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Available
Specificity	IgG, Fcγ fragment specific
Manufacturer - Category	F(ab')₂ Fragment Affinity-Purified Antibodies
Manufacturer - Conjugate / Tag	Horseradish Peroxidase
Storage Conditions	Store freeze-dried solid at 2-8°C. Rehydrate with the indicated volume of dH2O (see product specification sheet) and centrifuge if not clear. Prepare working dilution on day of use. Product is stable for about 6 weeks at 2-8°C as an undiluted liquid.
Expiration Date	one year from date of rehydration. The expiration date may be extended if test results are acceptable for the intended use.
Extended Storage after Rehydration	Aliquot and freeze at -70°C or below. Avoid repeated freezing and thawing. Alternatively, add an equal volume of glycerol (ACS grade or better) for a final concentration of 50%, and store at -20°C as a liquid.
Preservative	None (Warning: Use of sodium azide as a preservative will substantially inhibit the enzyme activity of horseradish peroxidase.)
Suggested Working Concentration or Dilution Range	1:500 - 1:5, 000 for immunohisto/cytochemistry 1:5, 000 - 1:100, 000 for ELISA and Western blotting with chromogenic substrates 1:10, 000 - 1:200, 000 for Western blotting with ECL substrates Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.
Buffer	0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6
Stabilizer	15 mg/ml Bovine Serum Albumin (IgG-Free, Protease-Free)
Manufacturer - Specificity	IgG, Fcγ fragment specific
Clonality	Polyclonal
Infomation	Based on immunoelectrophoresis and/or ELISA, the antibody reacts with the Fc portion of mouse IgG heavy chain but not with the Fab portion of mouse immunoglobulins. No antibody was detected against mouse IgM or against non-immunoglobulin serum proteins. The antibody may cross-react with immunoglobulins from other species.
Physical State	Freeze-dried solid
Purity	The antibody was purified from antisera by a combination of pepsin digestion and immunoaffinity chromatography using antigens coupled to agarose beads. Fc fragments and whole IgG molecules have been removed.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Request Data Sheet

115-036-008-datasheet.pdf

115-036-008-safety-datasheet.pdf