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Biotin-SP-conjugated AffiniPure-VHH® Fragment Alpaca Anti-Rabbit IgG (H+L) (min X Bov, Hu, Ms Sr Prot)

Biotin-SP-conjugated AffiniPure-VHH® Fragment Alpaca Anti-Rabbit IgG (H+L) (min X Bov, Hu, Ms Sr Prot)

Item no.	611-064-215
Manufacturer	Jackson ImmunoResearch
Amount	0,1 ml
Category	Antibodies Secondary Antibodies
Type	Antibody Secondary
Format	Lyophilized
Specific against	Rabbit (Oryctolagus cuniculus)
Host	Alpaca
Isotype	sdAb (VHH)
Conjugate/Tag	Biotin
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Available
Manufacturer - Category	AffiniPure-VHH™ Secondary Antibodies
Manufacturer - Conjugate / Tag	Biotin-SP (long spacer)
Storage Conditions	Store freeze-dried solid at 2-8°C. Rehydrate with the indicated volume of dH2O (see product specification sheet) and centrifuge if not clear. Prepare working dilution on day of use. Product is stable for about 6 weeks at 2-8°C as an undiluted liquid.
Expiration Date	one year from date of rehydration. The expiration date may be extended if test results are acceptable for the intended use.
Extended Storage after Rehydration	Aliquot and freeze at -70°C or below. Avoid repeated freezing and thawing. Alternatively, add an equal volume of glycerol (ACS grade or better) for a final concentration of 50%, and store at -20°C as a liquid.
Preservative	0.05% Sodium Azide
Suggested Working Concentration or Dilution Range	1:20, 000 - 1:400, 000 for ELISA and Western blotting using enzyme-conjugated streptavidin 1:500 - 1:5, 000 for enzyme immunohisto/cytochemistry 1:200 - 1:1, 000 for flow cytometry and fluorescence immunohisto/cytochemistry Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.
Buffer	0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6
Stabilizer	15 mg/ml Bovine Serum Albumin (IgG-Free, Protease-Free)
Manufacturer - Specificity	IgG (H+L)
Minimal Cross Reactivity	Bovine, Human, Mouse Serum Proteins
Infomation	Based on immunoelectrophoresis and/or ELISA, the antibody reacts with whole molecule rabbit IgG. It also reacts with the light chains of other rabbit immunoglobulins. The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with bovine, human and mouse serum proteins, but it may cross-react with immunoglobulins from other species.
Physical State	Freeze-dried solid
Purity	The antibody was purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads. Fc fragments and whole IgG molecules have been removed.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 0,1 ml

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Delivery expected until 3/7/2025