Listeriolysin-O Recombinant

Product Name: Listeriolysin-O Recombinant
Synonyms: Listeriolysin-O, LLO, hlyA.
Description: Molecular weight of Listeriolysin O is 58kDa. This haemolysin protein is provided in its mature form that is constituted by 503 amino acids, from 26 to 529 of the registered sequence (accession number: AAF64524).
Uniprot Accesion Number: Q9L5B9
Amino Acid Sequence:
Source: Escherichia Coli.
Physical Appearance and Stability: Store at 4C if entire vial will be used within 2-4 weeks. Store, frozen at -20C for longer periods of time. Avoid multiple freeze-thaw cycles.
Formulation and Purity: The protein contains 50mM NaH2PO4, 1mM EDTA, 2.7mM KCl, 1mM DTT, 5% (v/v)  glycerol and 0.5M NaCl. Greater than 90.0% as determined by SDS-PAGE.
Application: a. Cytosolic delivery of molecules, peptides, oligonucleodides and plasmid DNA (4). b. Production of specific monoclonal antibodies (5). c. Detection of anti-listeriolysin O antibodies (6).
Solubility:
Biological Activity: Hemolytic activity for Recombinant Listeriolysin O was 1.18 x 105  HU/mg of protein where HU means hemolytic activity unit that is the amount of toxin needed to release half the hemoglobin (50% lysis) of the erythrocytes as determined by hemolysin assay.2mM DTT is used to reactivate the toxin.
Shipping Format and Condition: Lyophilized powder at room temperature.
Usage Statement: Our products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.