Description |
Product Name: Beta-Nerve Growth Factor Human Recombinant, CHO Synonyms: Beta Polypeptide, NGF, NGFB, HSAN5, Beta-NGF, MGC161426, MGC161428. Description: Nerve Growth Factor-beta Human Recombinant produced in CHO is a noncovalently disulfide linked homodimer, glycosylated, polypeptide chain (Ser122-Arg239) containing 2 identical 118 amino acids and having a molecular mass of 26.5 kDa.The NGF-b is purified by proprietary chromatographic techniques. Uniprot Accesion Number: P01138 Amino Acid Sequence:Was analyzed by Mass spectrometry. Source: Chinese Hamster Ovary Cells. Physical Appearance and Stability: Lyophilized Nerve Growth Factor b although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution Nerve Growth Factor-beta should be stored at 4C between 2-7 days and for future use below -18C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles. Formulation and Purity: The protein was lyophilized from a 0.2um filtered solution in 20mM PB and 250mM NaCl, pH 7.0. Greater than 95.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE. Application: Solubility: It is recommended to reconstitute the lyophilized NGF-b in sterile 18MO-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions. Biological Activity: The ED50, calculated by its ability to stimulate chick E9 DRG neurite outgrowth was found to be < 1.0 ng/ml, corresponding to a specific activity of > 1 x 106 units/mg. Shipping Format and Condition: Lyophilized powder at room temperature. Usage Statement: Our products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. |