Description |
Product Name: Insulin-Like Growth Factor Binding Protein-4 Human Synonyms: Insulin-like growth factor-binding protein 4, IBP-4, IGF-binding protein 4, IGFBP-4, IGFBP4, IBP4, BP-4, HT29-IGFBP. Description: IGFBP-4 Human Recombinant amino acids Asp22- Glu258, produced in HEK293 cells and fused with a polyhistidine tag at the C-terminus. IGFBP4 predicted Mw is 27kDa and on SDS-PAGE appears as a 32kDa band under denaturing conditions.IGFBP4 is purified by proprietary chromatographic techniques. Uniprot Accesion Number: P22692 Amino Acid Sequence:DEAIHCPPCS EEKLARCRPP VGCEELVREP GCGCCATCAL GLGMPCGVYT PRCGSGLRCY PPRGVEKPLH TLMHGQGVCM ELAEIEAIQE SLQPSDKDEG DHPNNSFSPC SAHDRRCLQK HFAKIRDRST SGGKMKVNGA PREDARPVPQ GSCQSELHRA LERLAASQSR THEDLYIIPI PNCDRNGNFH PKQCHPALDG QRGKCWCVDR KTGVKLPGGL EPKGELDCHQ LADSFRE + His Tag. Source: HEK293 cells. Physical Appearance and Stability: Lyophilized IGFBP4 although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution IGFBP-4 should be stored at 4C between 2-7 days and for future use below -18C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles. Formulation and Purity: IGFBP 4 was lyophilized after extensive dialysis against PBS. Greater than 95.0% as determined by SDS-PAGE. Application: Solubility: It is recommended to reconstitute the lyophilized IGFBP4 in sterile 18M-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions. Biological Activity: The ED50 range is 0.01-0.09ug/ml as measured by its ability to inhibit the biological activity of IGFII (20ng/ml) on MCF7 human breast adenocarcinoma cells. Shipping Format and Condition: Lyophilized powder at room temperature. Usage Statement: Our products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. |