Dihydrofolate Reductase Mouse Recombinant

Product Name: Dihydrofolate Reductase Mouse Recombinant
Synonyms: Dihydrofolate reductase, DHFR, DHFRP1, AA607882, AI662710, AW555094, 8430436I03Rik.
Description: DHFR  Mouse Recombinant fused with a  20 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing  207 amino acids (1-187 a.a.) and having a molecular mass of 23.8kDa. The DHFR is purified by proprietary chromatographic techniques.
Uniprot Accesion Number: P00375
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MVRPLNCIVA VSQNMGIGKN GDLPWPPLRN EFKYFQRMTT TSSVEGKQNL VIMGRKTWFS IPEKNRPLKD RINIVLSREL KEPPRGAHFL AKSLDDALRL IEQPELASKV DMVWIVGGSS VYQEAMNQPG HLRLFVTRIM QEFESDTFFP EIDLGKYKLL PEYPGVLSEV QEEKGIKYKF EVYEKKD.
Source: Escherichia Coli.
Physical Appearance and Stability: Store at 4C if entire vial will be used within 2-4 weeks. Store, frozen at -20C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Formulation and Purity: The DHFR solution (1 mg/ml) contains 20mM Tris-HCl buffer(pH 8.0), 10% glycerol, 2mM DTT and 0.1M NaCl. Greater than 95.0% as determined by SDS-PAGE.
Application:
Solubility:
Biological Activity: Specific activity is > 0.2 units/mg, in which one unit will convert 1.0 umole of 7, 8 ¿dihydrofloate and beta-NADPH to 5, 6, 7, 8-tetrahydrofloate and beta-NADP per min at pH 6.5 at 25C.
Shipping Format and Condition: Lyophilized powder at room temperature.
Usage Statement: Our products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.