Sonic HedgeHog Human Recombinant

Product Name: Sonic HedgeHog Human Recombinant
Synonyms: SHH, HHG-1, HHG1, Sonic hedgehog protein, TPT, HLP3, HPE3, SMMCI, TPTPS, MCOPCB5.
Description: Sonic HedgeHog Recombinant Human produced in E.Coli is a single, non-glycosylated polypeptide chain containing 179 amino acids and having a molecular mass of 20.1kDa.  The Cys at position 2 has been substituted with 2 Ile¿s. 
Uniprot Accesion Number: Q15465
Amino Acid Sequence:MIIGPGRGFG KRRHPKKLTP LAYKQFIPNV AEKTLGASGR YEGKISRNSE RFKELTPNYN PDIIFKDEEN TGADRLMTQR CKDKLNALAI SVMNQWPGVK LRVTEGWDED GHHSEESLHY EGRALDITTS DRDRSKYGML ARLAVEAGFD WVYYESKAHI HCSVKAENSV AAKSGGCFP
Source: Escherichia Coli.
Physical Appearance and Stability: Lyophilized Human Sonic HedgeHog although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution Sonic HedgeHog should be stored at 4C between 2-7 days and for future use below -18C.Please prevent freeze-thaw cycles.
Formulation and Purity: SHH is lyophilized from 10mM Na3PO4, pH 7.5. Greater than 97.0% as determined by SDS-PAGE.
Application:
Solubility: It is recommended to reconstitute the lyophilized SHH in sterile 10mM Na3PO4 pH 7.5 not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
Biological Activity: The activity is measured by the dose-dependent induction of alkaline phosphatase production by C3H/10T1/2 (CCL-226) fibroblasts and is 1.2-1.8ug/ml corresponding to a specific activity of 800U/mg.
Shipping Format and Condition: Lyophilized powder at room temperature.
Usage Statement: Our products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.