Bone Morphogenetic Protein-7 Human Recombinant, CHO

Product Name: Bone Morphogenetic Protein-7 Human Recombinant, CHO
Synonyms: Osteogenic Protein 1, BMP-7.
Description: N-TERMINAL---Human BMP-2 (Met 1 ¿ Arg 282) Human BMP-7 (Ser 293 ¿ Arg 431)---C-TERMINAL. The DNA sequence encoding the human BMP-2 signal peptide and propeptide (1ca.282 amino acid) fused to the human rhBMP-7 mature chain (293ca.431 amino acid) was expressed in a Chinese hamster ovary cell line. The mature recombinant BMP-7 generated by the proteolytic removal of the signal peptide and propetide contains 139 amino acid residues. The glycosylation of BMP-7 increases the molecular mass and the glycosylated proteins migrate as 25 ca. 40 kDa in SDS-PAGE under non-reducing conditions. BMP-7 is purified by proprietary chromatographic techniques.
Uniprot Accesion Number: P18075
Amino Acid Sequence:
Source: Chinese Hamster Ovarian Cells.
Physical Appearance and Stability: Lyophilized BMP-7 although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution BMP 7 Human should be stored at 4C between 2-7 days and for future use below -18C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Formulation and Purity: BMP-7 was lyophilized from a concentrated (1mg/ml) sterile solution containing 1% sucrose, 1.2% mannitol, 20mM glycine and 0.05% polysorbate 20 pH-4.   Greater than 97.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.
Application:
Solubility: It is recommended to reconstitute the lyophilized Bone Morphogenetic Protein-7 in 0.5mg/1ml sterile/ endotoxin free water.
Biological Activity: Measured in alkaline phosphatase activity assay using MC3T3-E1 cells. The ED50 for this effect is < 70ng/ml, corresponding to a Specific Activity of 14, 286IU/mg.
Shipping Format and Condition: Lyophilized powder at room temperature.
Usage Statement: Our products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.