Goat anti-Pleiotrophin, Biotinylated Antibody

Goat anti-Pleiotrophin, Biotinylated Antibody
Principle Name: Pleiotrophin, Biotinylated
Official Symbol: PTN
All Name and Symbols: PTN; pleiotrophin; HARP; HBGF8; HBNF; NEGF1; HB-GAM; HBBM; HBGF-8; HBNF-1; OSF-1; heparin affin regulatory protein; heparin binding growth factor 8; heparin-binding brain mitogen; heparin-binding growth factor 8; heparin-binding growth-associated molecule
Accession Number(s): NP_002816.1
Peptide Sequence: KEGKKQEKMLD., is from C Terminus
Human Gene ID(s): 5764
Non-Human GeneID(s): 19242 (mouse); 24924 (rat);
Important Comments:
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA, WB, IHC
Tested Species: Human, Mouse
Peptide ELISA: antibody detection limit dilution 1 to 8000.
Western Blot: Approx 18kDa band observed in Mouse fetal Brain lysates (calculated MW of 18.9kDa according to Mouse NP_032999.1). See non-biotinylated parental product's datasheet for further QC data. Recommended concentration: 0.1-0.3ug/ml.
Reference(s): Chang Y, Zuka M, Perez-Pinera P, Astudillo A, Mortimer J, Berenson JR, Deuel TF. Secretion of pleiotrophin stimulates breast cancer progression through remodeling of the tumor microenvironment. Proceedings of the National Academy of Sciences of the United States of America 2007 Jun 104 (26): 10888-93..PMID: 17578909