Goat anti-PGK1 Antibody

Goat anti-PGK1 Antibody
Principle Name: PGK1
Official Symbol: PGK1
All Name and Symbols: PGK1; phosphoglycerate kinase 1; MIG10; PGKA; PRP 2; cell migration-inducing gene 10 protein; primer recognition protein 2
Accession Number(s): NP_000282.1
Peptide Sequence: SKKYAEAVTRAKQ, is from internal region
Human Gene ID(s): 5230
Non-Human GeneID(s):
Important Comments:
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA, WB
Tested Species: Human
Peptide ELISA: antibody detection limit dilution 1 to 128000.
Western Blot: Approx 45kDa band observed in lysates of cell lines A432, HEK293, HeLa and HepG2 (calculated MW of 44.6kDa according to NP_000282.1). Recommended concentration: 0.1-0.3ug/ml.
Reference(s): Wang J, Ying G, Wang J, Jung Y, Lu J, Zhu J, Pienta KJ, Taichman RS. Characterization of phosphoglycerate kinase-1 expression of stromal cells derived from tumor microenvironment in prostate cancer progression. Cancer research 2010 Jan 70 (2): 471-80..PMID: 20068185