Goat anti-contactin 1 Antibody

Goat anti-contactin 1 Antibody
Principle Name: contactin 1
Official Symbol: CNTN1
All Name and Symbols: CNTN1; contactin 1; F3; GP135; contactin-1; glycoprotein gP135; neural cell surface protein F3
Accession Number(s): NP_001834.2; NP_778203.1; NP_001242992.1
Peptide Sequence: ENIRGKDKHQAR, is from internal region
Human Gene ID(s): 1272
Non-Human GeneID(s): 12805 (mouse); 117258 (rat);
Important Comments: This antibody is expected to recognize all reported isoforms (NP_001834.2; NP_778203.1; NP_001242992.1). Reported variants represent identical protein: NP_001242993.1, NP_001242992.1.
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA, WB
Tested Species: Human, Mouse, Rat, Pig
Peptide ELISA: antibody detection limit dilution 1 to 32000.
Western Blot: Approx 140kDa band observed in Human, Mouse, Rat and Pig Brain lysates (calculated MW of 113kDa according to NP_001834.2). The observed molecular weight corresponds to the glycosylated form. Recommended concentration: 0.3-1ug/ml.
Reference(s): Lamprianou S, Chatzopoulou E, Thomas JL, Bouyain S, Harroch S. A complex between contactin-1 and the protein tyrosine phosphatase PTPRZ controls the development of oligodendrocyte precursor cells. Proc Natl Acad Sci U S A. 2011 Oct 18; 108(42):17498-503..PMID: 21969550