Goat anti-Synaptotagmin-3 (aa25-38) Antibody

Goat anti-Synaptotagmin-3 (aa25-38) Antibody
Principle Name: Synaptotagmin-3 (aa25-38)
Official Symbol: SYT3
All Name and Symbols: SYT3; synaptotagmin III; DKFZp761O132; SytIII; synaptotagmin-3
Accession Number(s): NP_115674.1
Peptide Sequence: RDADTNDRCQEFN, is from intenal region (near N Terminus)
Human Gene ID(s): 84258
Non-Human GeneID(s): 20981 (mouse); 25731 (rat);
Important Comments: Reported variants represent identical protein: NP_001153801.1, NP_001153800.1, NP_115674.1. The immunizing peptide represents part of the vesicular domain.
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA
Tested Species:
Peptide ELISA: antibody detection limit dilution 1 to 128000.
Western Blot: Preliminary experiments in Human, Mouse and Rat Brain lysates gave no specific signal but low background (at antibody concentration up to 1ug/ml). We would appreciate any feedback from people in the field - have any results been reported wi
Reference(s): Li C, Ullrich B, Zhang JZ, Anderson RG, Brose N, Südhof TC. Ca(2+)-dependent and -independent activities of neural and non-neural synaptotagmins. Nature. 1995 Jun 15; 375(6532):594-9..PMID: 7791877