Goat anti-PDCD4 Antibody

Goat anti-PDCD4 Antibody
Principle Name: PDCD4
Official Symbol: PDCD4
All Name and Symbols: PDCD4; programmed cell death 4 (neoplastic transformation inhibitor); H731; MGC33046; MGC33047; OTTHUMP00000020483; neoplastic transformation inhibitor protein; nuclear antigen H731; programmed cell death protein 4; protein 197/15a
Accession Number(s): NP_055271.2; NP_663314.1; NP_001186421.1
Peptide Sequence: DSYKGTVDCVQAR, is from internal region
Human Gene ID(s): 27250
Non-Human GeneID(s): 18569 (mouse); 64031 (rat);
Important Comments: This antibody is expected to recognize all reported isoforms (NP_055271.2; NP_663314.1; NP_001186421.1).
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA, WB
Tested Species: Human, Mouse
Peptide ELISA: antibody detection limit dilution 1 to 128000.
Western Blot: Approx 60kDa band observed in nuclear lysates of cell lines NIH3T3 and HeLa (calculated MW of 51.7kDa according to NP_055271.2). Recommended concentration: 1-3ug/ml.
Reference(s): Zhang X, Wang X, Song X, Liu C, Shi Y, Wang Y, Afonja O, Ma C, Chen YH, Zhang L. Programmed cell death 4 enhances chemosensitivity of ovarian cancer cells by activating death receptor pathway in vitro and in vivo. Cancer Sci. 2010 Oct; 101(10):2163-70..PMID: 20735432