Description |
Goat anti-MEX3C (aa541-554) Antibody Principle Name: MEX3C (aa541-554) Official Symbol: MEX3C All Name and Symbols: MEX3C; mex-3 homolog C (C. elegans); FLJ38871; MEX-3C; RKHD2; RNF194; OTTHUMP00000163545; RING finger and KH domain-containing protein 2; RING finger protein 194; RNA-binding protein MEX3C; ring finger and KH domain containing 2 Accession Number(s): NP_057710.3 Peptide Sequence: TPRLSPTFPESIEH, is from internal region Human Gene ID(s): 51320 Non-Human GeneID(s): 240396 (mouse); 307271 (rat); Important Comments: Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20C. Minimize freezing and thawing. Applications: Pep ELISA, WB Tested Species: Mouse Peptide ELISA: antibody detection limit dilution 1 to 32000. Western Blot: Approx 60+36kDa bands observed in Mouse Testis lysates, and these bands reduced after knock-down by gene trap (calculated MW of 69.4kDa according to NP_057710.3). Recommended concentration: 0.2-0.5ug/ml. Reference(s): Buchet-Poyau K, Courchet J, Le Hir H, Seraphin B, Scoazec JY, Duret L, Domon-Dell C, Freund JN, Billaud M. Identification and characterization of human Mex-3 proteins, a novel family of evolutionarily conserved RNA binding proteins differentially localized to processing bodies. Nucleic Acids Res. 2007; 35(4):1289-300..PMID: 17267406 |