Description |
Goat anti-Trpc6 (mouse) Antibody Principle Name: Trpc6 (mouse) Official Symbol: Trpc6 All Name and Symbols: Trpc6; transient receptor potential cation channel, subfamily C, member 6; AV025995; LLHWJM002; LLHWJM003; LLHWJM004; TRP-6; Trrp6; mtrp6; calcium entry channel; short transient receptor potential channel 6; transient receptor protein 6 Accession Number(s): NP_038866.2 Peptide Sequence: KQSSTRSSEDYHLNS, is from internal region Human Gene ID(s): Non-Human GeneID(s): 22068 (mouse); 89823 (rat); Important Comments: Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20C. Minimize freezing and thawing. Applications: Pep ELISA, IHC Tested Species: Human Peptide ELISA: antibody detection limit dilution 1 to 64000. Western Blot: Preliminary experiments gave an approx 75kDa band in Mouse and Rat Brain and Spinal Cord lysates after 0.3ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the Immunohistochemistry: Paraffin embedded Human Colon. Recommended concentration: 5ug/ml. Reference(s): Graham S, Gorin Y, Abboud HE, Ding M, Lee DY, Shi H, Ding Y, Ma R. Abundance of TRPC6 protein in glomerular mesangial cells is decreased by ROS and PKC in diabetes. Am J Physiol Cell Physiol. 2011 Aug; 301(2):C304-15..PMID: 21525431 |