Goat anti-SLC19A1 / RFC1 (mouse) Antibody

Goat anti-SLC19A1 / RFC1 (mouse) Antibody
Principle Name: SLC19A1 / RFC1 (mouse)
Official Symbol: Slc19a1
All Name and Symbols: Slc19a1; solute carrier family 19 (folate transporter), member 1; AI323572; RFC; RFC-1; RFC1; IFC-1; OTTMUSP00000045639; OTTMUSP00000045641; folate transporter 1; intestinal folate carrier 1; intestinal folate carrier protein; reduced folate carrier 1; so
Accession Number(s): NP_112473.1
Peptide Sequence: KQAYEEPRQDHELK, is from N Terminus
Human Gene ID(s):
Non-Human GeneID(s): 20509 (mouse); 29723 (rat);
Important Comments:
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA
Tested Species:
Peptide ELISA: antibody detection limit dilution 1 to 128000.
Western Blot: Preliminary experiments gave bands at approx 65kDa and 26kDa in Mouse Skin lysates after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size
Reference(s): Adjei AA, Salavaggione OE, Mandrekar SJ, Dy GK, Ziegler KL, Endo C, Molina JR, Schild SE, Adjei AA. Correlation between polymorphisms of the reduced folate carrier gene (SLC19A1) and survival after pemetrexed-based therapy in non small cell lung cancer: a North Central Cancer Treatment Group-based exploratory study. J Thorac Oncol. 2010 Sep; 5(9):1346-53..PMID: 20651609