Goat anti-HYPE / FICD Antibody

Goat anti-HYPE / FICD Antibody
Principle Name: HYPE / FICD
Official Symbol: FICD
All Name and Symbols: FICD; FIC domain containing; HIP13; HYPE; MGC5623; UNQ3041; Huntingtin interacting protein E; fic S-phase protein cell division homolog; huntingtin interacting protein 13; huntingtin interactor protein E
Accession Number(s): NP_009007.2
Peptide Sequence: PPITIRKEQRSD, is from internal region
Human Gene ID(s): 11153
Non-Human GeneID(s): 231630 (mouse); 288741 (rat);
Important Comments:
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA
Tested Species:
Peptide ELISA: antibody detection limit dilution 1 to 16000.
Western Blot: Preliminary experiments gave an approx 60kDa band in lysates of Mouse and Rat Liver and of cell line HepG2 after 0.3ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe
Reference(s): Clark HF, Gurney AL, Abaya E, Baker K, Baldwin D, Brush J, Chen J, Chow B, Chui C, Crowley C, Currell B, Deuel B, Dowd P, Eaton D, Foster J, Grimaldi C, Gu Q, Hass PE, Heldens S, Huang A, Kim HS, Klimowski L, Jin Y, Johnson S, Lee J, Lewis L, Liao D, Mark The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment. Genome research 2003 Oct 13 (10): 2265-70..PMID: 12975309