Description |
Goat anti-Transcortin / CBG (rat) Antibody Principle Name: Transcortin / CBG (rat) Official Symbol: Serpina6 All Name and Symbols: Serpina6; serine (or cysteine) peptidase inhibitor, clade A, member 6; MGC112780; Cbg; corticosteroid binding globulin; corticosteroid-binding globulin; serine (or cysteine) proteinase inhibitor, clade A, (alpha-1 antiproteinase, antitrypsin) member 6; se Accession Number(s): NP_001009663.1 Peptide Sequence: KASQQINQHVKDKT, is from internal region Human Gene ID(s): Non-Human GeneID(s): 299270 (rat); Important Comments: Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20C. Minimize freezing and thawing. Applications: Pep ELISA, WB Tested Species: Rat Peptide ELISA: antibody detection limit dilution 1 to 16000. Western Blot: Approx 55kDa band observed in Rat Liver lysates (calculated MW of 44.7kDa according to NP_001009663.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Richard et al, Endocrinology. 201 Reference(s): Braun BC, Meyer HA, Reetz A, Fuhrmann U, Köhrle J. Effect of mutations of the human serpin protein corticosteroid-binding globulin on cortisol-binding, thermal and protease sensitivity. The Journal of steroid biochemistry and molecular biology 2010 May 120 (1): 30-7..PMID: 20226861 |