Goat anti-SDF4 (aa161-175) Antibody

Goat anti-SDF4 (aa161-175) Antibody
Principle Name: SDF4 (aa161-175)
Official Symbol: SDF4
All Name and Symbols: SDF4; stromal cell derived factor 4; Cab45; 45 kDa calcium-binding protein; OTTHUMP00000001754; SDF-4; calcium binding protein; stromal cell-derived factor 4
Accession Number(s): NP_057631.1; NP_057260.2
Peptide Sequence: EYKVKFLASKGHSEK, is from internal region
Human Gene ID(s): 51150
Non-Human GeneID(s): 20318 (mouse); 155173 (rat);
Important Comments: This antibody is expected to recognize both reported isoforms (NP_057631.1; NP_057260.2).
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA, WB, IHC
Tested Species: Human
Peptide ELISA: antibody detection limit dilution 1 to 64000.
Western Blot: Approx 45kDa band observed in lysates of cell line Jurkat (calculated MW of 41.8kDa according to NP_057260.2). Recommended concentration: 0.1-0.3ug/ml. Primary incubation was 1 hour. Preliminary testing was unsuccessful on Mouse for this pa
Immunohistochemistry: In paraffin embedded Human Kidney shows preferential staining in the brush borders of epithelial cells. Recommended concentration: 5-10ug/ml.
Reference(s): Kang H, Escudero-Esparza A, Douglas-Jones A, Mansel RE, Jiang WG, Transcript analyses of stromal cell derived factors (SDFs): SDF-2, SDF-4 and SDF-5 reveal a different pattern of expression and prognostic association in human breast cancer. International journal of oncology 2009 Jul 35 (1): 205-11..PMID: 19513569