Goat anti-RUFY1 Antibody

Goat anti-RUFY1 Antibody
Principle Name: RUFY1
Official Symbol: RUFY1
All Name and Symbols: RUFY1; RUN and FYVE domain containing 1; FLJ22251; RABIP4; ZFYVE12; FYVE-finger protein EIP1; OTTHUMP00000223250; RUN and FYVE domain-containing 1; RUN and FYVE domain-containing protein 1; la-binding protein 1; rab4-interacting protein; zinc finger FYVE
Accession Number(s): NP_079434.3; NP_001035541.1
Peptide Sequence: QKNEAITSFEGKT, is from internal region
Human Gene ID(s): 80230
Non-Human GeneID(s): 216724 (mouse); 360521 (rat);
Important Comments: This antibody is expected to recognize both reported isoforms (NP_079434.3; NP_001035541.1). Reported variants represent identical protein: NP_001035542.1, NP_001035541.1.
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA, WB
Tested Species: Human
Peptide ELISA: antibody detection limit dilution 1 to 32000.
Western Blot: Approx 85kDa band observed in lysates of cell line HeLa (calculated MW of 79.8kDa according to NP_079434.3). Recommended concentration: 0.05-0.2ug/ml.
Reference(s): Matsuoka S, Ballif BA, Smogorzewska A, McDonald ER, Hurov KE, Luo J, Bakalarski CE, Zhao Z, Solimini N, Lerenthal Y, Shiloh Y, Gygi SP, Elledge SJ, ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage. Science (New York, N.Y.) 2007 May 316 (5828): 1160-6..PMID: 17525332