Description |
Goat anti-PEBP4 Antibody Principle Name: PEBP4 Official Symbol: PEBP4 All Name and Symbols: PEBP4; phosphatidylethanolamine-binding protein 4; CORK-1; CORK1; GWTM1933; MGC22776; PRO4408; cousin-of-RKIP 1 protein Accession Number(s): NP_659399.2 Peptide Sequence: RERASEPKHKNQAE, is from C Terminus Human Gene ID(s): 157310 Non-Human GeneID(s): Important Comments: Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20C. Minimize freezing and thawing. Applications: Pep ELISA, WB, IHC Tested Species: Human Peptide ELISA: antibody detection limit dilution 1 to 4000. Western Blot: Approx 26kDa band observed in Human Heart lysates (calculated MW of 25.7kDa according to NP_659399.2). Recommended concentration: 1-3ug/ml. An additional band of unknown identity was also consistently observed at 30kDa. This band was succe Immunohistochemistry: In paraffin embedded Human Pancreas shows staining consistent with lysosomes. Recommended concentration, 5-10ug/ml. Reference(s): Trynka G, Zhernakova A, Romanos J, Franke L, Hunt K, Turner G, Platteel M, Ryan AW, de Kovel C, Barisani D, Bardella MT, McManus R, Van Heel DA, Wijmenga C. Coeliac disease associated risk variants in TNFAIP3 and REL implicate altered NF-{kappa}B signalling. Gut. 2009 Feb 24. [Epub ahead of print].PMID: 19240061 |