Description |
Goat anti-EML5 Antibody Principle Name: EML5 Official Symbol: EML5 All Name and Symbols: EML5; echinoderm microtubule associated protein like 5; DKFZp781D1122; EMAP-2; HuEMAP-2 Accession Number(s): NP_899243.1 Peptide Sequence: QQKEPSIDERQG, is from internal region Human Gene ID(s): 161436 Non-Human GeneID(s): 319670 (mouse); 444982 (rat); Important Comments: Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20C. Minimize freezing and thawing. Applications: Pep ELISA, IHC Tested Species: Human Peptide ELISA: antibody detection limit dilution 1 to 32000. Western Blot: Preliminary experiments in Human Brain (Cerebellum, Cerebral Cortex and Hippocampus) lysates gave no specific signal but low background (at antibody concentration up to 1ug/ml). Immunohistochemistry: Paraffin embedded Human Small Intestine. Recommended concentration: 5ug/ml. Reference(s): O'Connor V, Houtman SH, De Zeeuw CI, Bliss TV, French PJ. Eml5, a novel WD40 domain protein expressed in rat brain. Gene 2004 Jul 336 (1): 127-37..PMID: 15225882 |