Goat anti-SIGLEC8 Antibody

Goat anti-SIGLEC8 Antibody
Principle Name: SIGLEC8
Official Symbol: SIGLEC8
All Name and Symbols: SIGLEC8; sialic acid binding Ig-like lectin 8; MGC59785; SAF2; SIGLEC-8; SIGLEC8L
Accession Number(s): NP_055257.2
Peptide Sequence: SYKSQLNYKTKQ, is from internal region
Human Gene ID(s): 27181
Non-Human GeneID(s):
Important Comments:
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA, WB
Tested Species: Human
Peptide ELISA: antibody detection limit dilution 1 to 32000.
Western Blot: Approx. 45kDa band observed in lysates of cell line MOLT4 (calculated MW of 54.0kDa according to NP_055257.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Nutku at al, Blood. 2003 J
Reference(s): Yokoi H, Choi OH, Hubbard W, Lee HS, Canning BJ, Lee HH, Ryu SD, von Gunten S, Bickel CA, Hudson SA, Macglashan DW Jr, Bochner BS. Inhibition of FcepsilonRI-dependent mediator release and calcium flux from human mast cells by sialic acid-binding immunoglobulin-like lectin 8 engagement. J Allergy Clin Immunol. 2008 Feb; 121(2):499-505.e1. Epub 2007 Nov 26..PMID: 18036650