Goat anti-ATG16L1 Antibody

Goat anti-ATG16L1 Antibody
Principle Name: ATG16L1
Official Symbol: ATG16L1
All Name and Symbols: ATG16 ; autophagy related 16-like 1 (S. cerevisiae) ; hCG_1817841; APG16L; ATG16L; FLJ00045; FLJ10035; FLJ10828; FLJ22677; WDR30; APG16 autophagy 16-like; APG16L beta; ATG16 autophagy related 16-like protein 1; WD repeat domain 30
Accession Number(s): NP_110430.5; NP_060444.3
Peptide Sequence: KVEKVLSKQHSSSIN, is from internal region (near the C Terminus)
Human Gene ID(s): 55054
Non-Human GeneID(s):
Important Comments: This antibody is expected to recognise both reported isoforms (NP_110430.5 and NP_060444.3).
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA, WB, IHC
Tested Species: Mouse, Human
Peptide ELISA: antibody detection limit dilution 1 to 32000.
Western Blot: Approx 65kDa band observed in Mouse Brain lysates (calculated MW of 68.1kDa according to Mouse NP_084122.2). Recommended concentration: 0.01-0.1ug/ml. Primary incubation was 1 hour.
Immunohistochemistry: Paraffin embedded Human Skin. Recommended concentration: 3.75ug/ml.
Reference(s): Rioux JD, Xavier RJ, Taylor KD, Silverberg MS, Goyette P, Huett A, Green T, Kuballa P, Barmada MM, Datta LW, Shugart YY, Griffiths AM, Targan SR, Ippoliti AF, Bernard EJ, Mei L, Nicolae DL, Regueiro M, Schumm LP, Steinhart AH, Rotter JI, Duerr RH, Cho JH, Genome-wide association study identifies new susceptibility loci for Crohn disease and implicates autophagy in disease pathogenesis. Nat Genet. 2007 May; 39(5):596-604. Epub 2007 Apr 15..PMID: 17435756