Goat anti-Frizzled 4 Antibody

Goat anti-Frizzled 4 Antibody
Principle Name: Frizzled 4
Official Symbol: FZD4
All Name and Symbols: Frizzled 4; frizzled homolog 4 (Drosophila); FZD4; EVR1; FEVR; FZD4S; Fz-4; FzE4; GPCR; MGC34390; WNT receptor frizzled-4; exudative vitreoretinopathy 1 (autosomal dominant Criswick-Schepens syndrome); frizzled 4
Accession Number(s): NP_036325.2
Peptide Sequence: DEEERRCDPIRIS, is from internal region
Human Gene ID(s): 8322
Non-Human GeneID(s): 64558 (rat);
Important Comments:
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA, WB
Tested Species: Human
Peptide ELISA: antibody detection limit dilution 1 to 16000.
Western Blot: Approx 60kDa band observed in Human Colon and Small Intestine (Ileum) lysates (calculated MW of 59.9kDa according to NP_036325.2). Recommended concentration: 0.3-1ug/ml.
Reference(s): You J, Nguyen AV, Albers CG, Lin F, Holcombe RF. Wnt Pathway-Related Gene Expression in Inflammatory Bowel Disease. Dig Dis Sci. 2007 Oct 16; [Epub ahead of print].PMID: 17939044