Description |
Goat anti-NEDD9 Antibody Principle Name: NEDD9 Official Symbol: NEDD9 All Name and Symbols: NEDD9; neural precursor cell expressed, developmentally down-regulated 9; CAS-L; CASL; HEF1; dJ49G10.2; dJ761I2.1; Crk-associated substrate related; cas-like docking dJ49G10.2 (Enhancer of Filamentation 1 (HEF1)); dJ761I2.1 (enhancer of filamentation (HEF Accession Number(s): NP_006394.1; NP_892011.2 Peptide Sequence: NKPQNKCDDLDR, is from internal region Human Gene ID(s): 4739 Non-Human GeneID(s): 18003 (mouse); 291044 (rat); Important Comments: This antibody is expected to recognise isoform 1 (NP_006394.1) only. Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20C. Minimize freezing and thawing. Applications: Pep ELISA, WB Tested Species: Human Peptide ELISA: antibody detection limit dilution 1 to 8000. Western Blot: Approx 100kDa band observed in Human Kidney, Lung and Placenta lysates (calculated MW of 92.9kDa according to NP_006394.1). Recommended concentration: 0.1-0.3ug/ml. Reference(s): Kim M, Gans JD, Nogueira C, Wang A, Paik JH, Feng B, Brennan C, Hahn WC, Cordon-Cardo C, Wagner SN, Flotte TJ, Duncan LM, Granter SR, Chin L. Comparative oncogenomics identifies NEDD9 as a melanoma metastasis gene. Cell. 2006 Jun 30; 125(7):1269-81..PMID: 16814714 |