Description |
Goat anti-PIWI / HIWI Antibody Principle Name: PIWI / HIWI Official Symbol: PIWIL1 All Name and Symbols: PIWIL1; HIWI; PIWI; piwi-like 1 (Drosophila); piwi (Drosophila)-like 1; homolog of Drosophila piwi; MIWI; piwi homolog; piwi-like 1 Accession Number(s): NP_004755.2; NP_001177900.1 Peptide Sequence: SNRKDKYDAIKKY, is from internal region Human Gene ID(s): 9271 Non-Human GeneID(s): 57749 (mouse); Important Comments: This antibody is expected to recognise both reported isoforms (NP_004755.2; NP_001177900.1). Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20C. Minimize freezing and thawing. Applications: Pep ELISA, IHC Tested Species: Human Peptide ELISA: antibody detection limit dilution 1 to 64000. Western Blot: Preliminary experiments showed a 100kDa band but also bands at approx 35kDa and 26-28kDa in Human Ovary, Placenta, Prostate and Testis lysates after 0.1ug/ml antibody staining. Please note that currently we cannot find an explanation in the Immunohistochemistry: In paraffin embedded Human Testis shows textured cytoplasm staining in all germ cells. Recommended concentration, 4-6ug/ml. Reference(s): Szakmary A, Cox DN, Wang Z, Lin H. Regulatory Relationship among piwi, pumilio, and bag-of-marbles in Drosophila Germline Stem Cell Self-Renewal and Differentiation. Curr Biol. 2005 Jan 26; 15(2):171-8. .PMID: 15668175 |