Goat anti-Semaphorin 5A Antibody

Goat anti-Semaphorin 5A Antibody
Principle Name: Semaphorin 5A
Official Symbol: SEMA5A
All Name and Symbols: SEMA5A; sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A; HGNC:10736; SEMAF; semF; sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane
Accession Number(s): NP_003957.1
Peptide Sequence: DEATKKACYSKGKS, is from internal region
Human Gene ID(s): 9037
Non-Human GeneID(s): 20356 (mouse); 310207 (rat);
Important Comments: The immunizing peptide corresponds to a part of the extracellular domain.
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA, IHC
Tested Species: Human
Peptide ELISA: antibody detection limit dilution 1 to 128000.
Western Blot: Preliminary experiments gave an approx 90kDa in Mouse Brain lysates and approx 25kDa band in Human Brain (Cerebellum), Colon, Heart and Kidney lysates after 0.03ug/ml antibody staining. Please note that currently we cannot find an explanati
Immunohistochemistry: In paraffin embedded Human Kidney shows membanous and vesicular staining in PCT cells. Recommended concentration: 4-6ug/ml.
Reference(s): Maraganore DM, de Andrade M, Lesnick TG, Strain KJ, Farrer MJ, Rocca WA, Pant PV, Frazer KA, Cox DR, Ballinger DG. High-resolution whole-genome association study of Parkinson disease Am J Hum Genet. 2005 Nov; 77(5):685-93. Epub 2005 Sep 9..PMID: 16252231