Description |
Goat anti-STELLAR Antibody Principle Name: STELLAR Official Symbol: STELLAR All Name and Symbols: STELLAR; germ and embryonic stem cell enriched protein STELLA ; developmental pluripotency associated 3 pseudogene, stella-like; STELLA; Accession Number(s): NP_954980.1 Peptide Sequence: NKEPKGVKKESRP, is from internal region Human Gene ID(s): 400206 Non-Human GeneID(s): Important Comments: Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20C. Minimize freezing and thawing. Applications: Pep ELISA Tested Species: Peptide ELISA: antibody detection limit dilution 1 to 64000. Western Blot: Preliminary experiments gave an approx 26kDa band in human ovary and placenta lysates after 0.1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated Reference(s): Ezeh UI, Turek PJ, Reijo RA, Clark AT. Human embryonic stem cell genes OCT4, NANOG, STELLAR, and GDF3 are expressed in both seminoma and breast carcinoma. Cancer. 2005 Nov 15; 104(10):2255-65. .PMID: 16228988 |