Goat anti-RASA3 / GAP1IP4BP Antibody

Goat anti-RASA3 / GAP1IP4BP Antibody
Principle Name: RASA3 / GAP1IP4BP
Official Symbol: RASA3
All Name and Symbols: RASA3; GAPIII; MGC46517; MGC47588; GAP1IP4BP; RAS p21 protein activator 3; GTPase activating protein III; Ins(1, 3, 4, 5)P4-binding protein; inositol 1, 3, 4, 5-tetrakisphosphate-binding protein; RP11-245B11.3
Accession Number(s): NP_031394.2
Peptide Sequence: QYKRDKFKKTKYGSQ, is from internal region (near the C Terminus)
Human Gene ID(s): 22821
Non-Human GeneID(s):
Important Comments:
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA
Tested Species:
Peptide ELISA: antibody detection limit dilution 1 to 32000.
Western Blot: Preliminary experiments in Human Brain lysates gave no specific signal but low background (at antibody concentration up to 1ug/ml). - have any results been reported with other antibodies/lysates?
Reference(s): Kuai J, Wooters J, Hall JP, Rao VR, Nickbarg E, Li B, Chatterjee-Kishore M, Qiu Y, Lin LL. NAK is recruited to the TNFR1 complex in a TNFalpha-dependent manner and mediates the production of RANTES: identification of endogenous TNFR-interacting proteins by a proteomic approach. J Biol Chem. 2004 Dec 17; 279(51):53266-71. Epub 2004 Oct 13. .PMID: 15485837